November 9, 2020
Study summary
- Using Synthace, we evaluated the Dynamic Binding Capacity (DBC) of two different resins packed in 200 µL RoboColumns® on a Tecan Freedom EVO® liquid handler, at different residence times
- Synthace structured A280 nm absorbance data from the plate reader, automatically created a pseudo-chromatogram and normalized the data by applying pathlength and blank correction.
- Using Synthace's data download capability, the normalized data was subsequently imported in Python to perform further smoothing and DBC analysis
Key findings
- MabSelect™ PrismA and Praesto® Jetted A50 resins have comparable DBC at shorter residence times
- Synthace enables flexible programming of RoboColumn® workflows, allowing users to specify residence times and fractions collected per individual column. Synthace workflows can be shared between teams to facilitate knowledge transfer and reduce experimental design time...
- Synthace promotes higher quality data by using unique identifiers for each experiment, structuring data in common formats, and keeping track of experimental metadata
...
Other posts you might be interested in
View All Posts
Data
Miniaturized Purification with Synthace and Tecan Te-Chrom™: Uniting Robust Execution With Flexible Planning and Comprehensive Data Analysis
5 min
Platform updates
Synthace just got a whole lot better: 4 improvements biologists need to hear about
11 min
Lab of the Future
A life sciences platform with flexibility at its core
8 min