Optimizing Construct Assembly with Synthace

Key findings

• Synthace improved the robustness and reproducibility of Design of Experiments (DOE) optimization of BsaI DNA-assembly protocols, ensuring >95% correct 7-part DNA assemblies
  
• The optimized experimental workflow was initially programmed for a Gilson PIPETMAX® liquid handler and easily transferred to a Hamilton Microlab® STAR, FORMULATRIX® MANTIS®, and Tecan Freedom EVO® robots, achieving comparable robustness
• The automated Synthace approach reduced time spent per experiment from 29.5 hours to 8.5 hours, resulting in a 21-hour time saving; up to 63 hours were saved across the whole study
• Overall, Synthace achieved a 74% time-saving in experimental planning, design, and execution. This means large efficiency savings in FTE time and a 22% reduction in tip usage